MINAFAB Characterization equipments

Microarray ploter - GeneMachines OmniGrid Micro

Technical characteristics:

Ploter microarray (GeneMachines OmniGrid Micro) is designed for producing DNA or protein microarrays on slides. Omni Grid Micro Plotter can dip into a source plate and spot a given volume of sample solution onto a solid surface (e.g. glass slide, silicon substrate). A Control Computer assures the utilization interface. A vacuum wash station ensures active washing in between sample transfers while humidity control minimizes evaporation of precious sample.
-x/y resolution: 1 1μm;
-available pins: 50, 100, 200 μm ;
-humidity control during processing;
-flexibility in array configuration;

Description

Protein microarray - Depending on the field of application, protein microarrays can be classified into two categories:
(1) Arrays for proteomics or focused protein profiling. This category can be subdivided by array format into forward- and reverse-phase protein microarrays. The difference between the two refers to the way the sample is applied. On forward-phase protein arrays, the sample is incubated on the array so that different analytes can be detected simultaneously. Examples include antibody microarrays that are used for the identification and
quantification of target proteins. On reverse-phase arrays different samples that are immobilised on a chip, in a single step, a large collection of probes can be screened for the presence or absence of one distinct target protein
Protein microarray is used to identify protein-protein interactions, to identify the substrates of protein kinases, or to identify the targets of biologically active small molecules.
(2) arrays for functional studies. Low and high density arrays of proteins, peptides and small molecules could be
used to study the binding of DNA, RNA, small chemical ligands and proteins to their immobilised binding partners.
DNA microarray
A typical microarray experiment compares gene expression levels in two different physiological conditions, for instance one can compare the same bacterial strain grown with or without antibiotics. In this case, nucleic acids of both samples are labeled with distinct fluorescent dyes, most popular dyes are Cy-3 (green) and Cy-5 (red).

******************************************************************

Applications:

Protein Arrays
- study tens of thousands of proteins in as short a time as possible;
- producing high density protein arrays on specialized slides;
- automated hybridization and imaging of cDNA and oligonucleotides now allow the consistent high throughput study of antibody/antigen interactions;
Protein Assays
- immuno-assays, protein-protein interaction assays, enzyme assays;  

Application procedure
- For each new application it is necessary to be specified the material that should be printed and the spot configuration in order to elaborate the software program;
- A preliminary accept is necessary to avoid the contamination risk;

******************************************************************

Results:

  • In microarray area results are related to functionalization of different surfaces (glass and silicon wafer) in order to immobilized DNA and Proteins used in medical diagnosis. We have optimised the process parameters in order to obtain a uniform hydrophilic/hydrophobic surface, high signal intensity and low background, spot with well defined morphology, and high efficiency with lower cost of slides and chemicals.
  • Enzyme-based sensors designed for electrical measurements of several toxins. The sensors are made of interdigital electrodes built on silicon substrate. Biomaterials are deposited on the electrode surface and are characterized using the NANOBIOLAB equipments.

    

 

Partneship:

  • Multialergen biochip realised by microarray technology, National Project, Program: Parteneriate. Main Partners: University of Bucharest, Faculty of Chemistry, Diagnostic SA (DDS), Genetic Lab.
******************************************************************

Application scientist: phys. Monica Simion, monica.simion@imt.ro

HOME

top of page

Last update: March 06, 2012