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Lucrari sustinute in cadrul proiectului:

Brevet

„Procedeu de realizare a unui biochip cu functia de amplificare a unor fragmente specifice de ADN prin reactia polimerazica in lant”, Monica Simion, Irina Kleps, Anca Angelescu, Teodora Neghina, Mihaela Miu, Adina Bragaru, Tatiana Oana Nedelcu, Florea Craciunoiu, (IMT-Bucuresti), Eduard Condac (UB, Facultatea de Biologie)

Inventia se refera la un procedeu de realizare a unui biochip pe substrat de siliciu care poate inlocui masina clasica de amplificare a unor fragmente specifice de ADN prin reactia polimerazica in lant (PCR), cat si sistemul de electroforeza capilara de analiza a materialului genetic. Procedeul se refera la realizarea unui microreactor de capacitate 2-4 ml, pe substrat de Si de orientare <100 > functionalizat cu nanoparticule de aur , cu orientarea predominanta <111>. Pe spatele microreactorului sunt realizate doua rezistente din Ti/Pt, una de 200-250 ohm, cu rol de incalzire, si alta de 50-70 ohm , cu rol de senzor de temperatura; aceste rezistente sunt comandate de un microcontroler in scopul realizarii ciclurilor termice necesare multiplicarii fragmentelor de ADN. Biochip-ul propus are un capac din sticla sau din elastomer de tip polidimetilsiloxan. Acest capac impiedica evaporarea reactantilor si a produsilor de reactie in timpul ciclului termic si permite analizarea prin microscopie de fluorescenta a materialului biologic. Capacul microreactoarelor se fixeaza cu sticla solubila.

Lucrari Ştiinţifice cotate ISI:

  • Microfluidics Silicon Structure for Electrophoresis Separation of DNA Fragments
    Monica Simion, Irina Kleps, Florea Craciunoiu, Lorand Savu, Mihaela Miu, and Adina Bragaru, Sensor Lett. 6, 585–589 (2008);
  • “Development of a new microfluidic analysis system based on porous silicon as sensitive element”, Mihaela Miu, A. Angelescu,  I. Kleps, S. Petrescu,  L. Zdrentu,  M. Simion, T. Ignat, Physica Status Solidi (c) 4, No. 6 (2007), pp. 2093-2097;
  • Irina Kleps, Mihai Danila, Anca Angelescu, Mihaela Miu, Monica Simion, Teodora Ignat, Adina Bragaru, Lucia Dumitru and Gabriela Teodosiu, “Gold and silver/Si nanocomposite layers”, “Materials Science and Engineering: C” Volume 27, Issues 5-8, 1439-1443, 2007
  • “Development of the micro- and nanoelectrodes for cells investigation”, Irina Kleps, Mihaela Miu, Florea Craciunoiu, Monica Simion, Adina Bragaru, Microelectronic Engineering, 94, 5-8, pp. 1744-1748;
  • “Nanoporous silicon matrix used as biomaterial”, Monica Simion, Irina Kleps, Teodora Negina, Anca Angelescu, Mihaela Miu, Adina Bragaru, Mihai Danila, Eduard Condac, Marieta Costache, Lorand Lavu, Journal of Alloys and Compounds (JALCOM), 434-435 (2007), pp. 830-832

Comunicari stiintifice:

„Study of the Au/ porous silicon substrate for organic molecules biosensing”, Irina Kleps, Mihaela Miu, Monica Simion, Teodora Ignat, Adina Bragaru, 6th Int. Conf. Inorganic Materials, 27.09.2008- 1.10.2008, Dresda, Germania;

Silicon surface functionalization for biomolecules attachment”, Adina Bragaru , Mihaela Miu, Monica Simion, Teodora Ignat, Irina Kleps, Andrei Avram, Veronica Schiopu, Florin Craciunoiu, poster prezentat la “1st Workshop- Polymers in Nanotechnology”, 26- 30.09.2007

„Non contact laser seeckle sensor for measuring one and two dimensional angular displacement D.Ulieru, E.Ulieru, A.Matei, A.Tantau „3nd International Conference ,,Smart materials structures systems``, CIMTEC 2008, June 8/13,2008, Arcireale Italy;

„High density interconnections fabrication by UV laser microprocessing of microvias and microstructures”, D.Ulieru,A.Matei,E.Ulieru, A.Tantau, F.Babarada 4M 2008 Multi Material Micro Manufacture 9/11 Sep.2008 Cardiff/UK;

Hydrophilic transformation of carbon films prepared by thermionic vacuum arc method, A. M. Lungu, P. Chiru, O. Pompilian, E.Dutu, C. P. Lungu, F. Craciunoiu, M. Simion, I. Kleps, G. Prodan, R. Vladoiu, V. Ciupina and G. Musa, Proc. of 18th International Symposium on Plasma Chemistry, August 26 - 31, 2007 - Kyoto, Japan;

Magnetic particles-based immunoassay for micotoxines determination”, M. Tudorache and C.Bala, LaborQ – Faculty of Chemistry, University of Bucharest, Sos. Panduri 90, 050663, Bucharest – 5, Romania, tel: + 40 21 4103178/int. 152, fax: + 40 21 4104888;

“Development of biosensors as analytical tools for environmental monitoring”, C.Bala, International Conference on Materials Science and Engineering- BRAMAT 2007;

‘Biophysical studies of light interaction with membrane model systems based on Chlorophyll A’, Laura Tugulea, Faculty of Physics - University of Bucharest, Proceedings of  4th International Workshop "Biological Effects of EMFs" 16 - 20 October 2006, Limin Hersonissou, Crete, Greece, vol I. pag. 147-154;

“Order changes in cell membrane as suggested mechanism for effects induced by applied electromagnetic field”E. Kovacs, T. Savopol, D. Martin, N. Iacob – XIXth  International Symposium on BIOELECTROCHEMISTRY and BIOENERGETICS – Oral Presentation,  page 142 – Abstract Book, Toulouse, FRANCE, April 2007;

“Fluorescent measurements of cell membrane potential under GSM electromagnetic fields exposure”Moisescu M.G., Leveque P., Mir L.M. – XIXth  International Symposium on BIOELECTROCHEMISTRY and BIOENERGETICS – Oral Presentation,  page 143 – Abstract Book, Toulouse, FRANCE, April 2007;

“Viability of murine melanocytes exposed to 2.45 GHz pulsed irradiation”N. Iacob, M. Moisescu, L. Vija, E. Boldoi, D. Martin, E. Kovacs – XIXth  International Symposium on BIOELECTROCHEMISTRY and BIOENERGETICS – Poster Presentation,  page 152 – Abstract Book, Toulouse, FRANCE, April 2007;

“Modeling the drug delivery by lipid vesicles into malignant cells”Iurie Paraico, Laura Bajenaru, Marius Surleac, Tudor Savopol, Eugenia Kovacs – International Autumn School “BIOPHYSICS FOR MEDICINE”, October 2007 – Poster Presentation (P-15), page 39 – Abstract Book, Mangalia, ROMANIA;

“Force calibration of optical trap by dielectrophoresis”T. Savopol, E. Papagiakoumou, M. Chirila, M. Makropoulou, A. Serafetinides, M. Radu, D. Pietreanu, G. Martinez-Lopez, M. Sancho Ruiz, E. Kovacs – 4th International Workshop “BIOLOGICAL EFFECTS of EMFs”, Oct.2006 – Oral Presentation, page 768 – Abstract Book, Crete, GREECE;

New methods proposed for evaluation of photoreceptor cells viability in retinal transplant”E. Kovacs, Tudor Savopol, Basarab Hosu – Gordon Conference “BIOELECTROCHEMISTRY” – Poster Presentation, Hot Topics section – Abstract Book, Aussois, FRANCE, sept. 2006;
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