Expected results

- Design of micro-electro-fluidic system that integrates components such as microchannels network, inlet / outlet chambers, electrodes system and electrical circuits;
- Virtual models using dedicated software packages, for testing and optimization of design specifications and of components functionalities;
- Techniques for modeling and coupled-field simulation that are basis of microsystem functionality;
- Masks design and fabrication, technological design of microfluidic system and electrical components;
- Experimental model of microsystem;
- Microphysical characterization of experimental structures;
- Experimental testing of cells separation and elecroporation;
- Evaluation of microsystem by electroporation efficiency point of view, by electrical and optical methods

- Synthesis, oriented functionalization and characterization of ferromagnetic particles used for cells caption and detection
- Preparing cells to be used in experiments and in-vitro characterization of dielectric and biophysical properties before and after electroporation;
- Synthesis and characterization of bioconjugates (ferromagnetic particles with specific cellular antibodies);
- Cells quantification by flow cytometry;
- Correlation and interpretation of results obtained by electrical detection.

- Methods for optical detection and quantification of electroporated cells;
- Experimental tests of separation and electroporation based on optical detection by holographic recording;
- Reconstruction of holographyc images, image processing to obtain morphological and structural parameters of cells
- Correlation and interpretation of results obtained by optical detection