The target of IMUNOSENSE project is the development of the technology of the fabrication of immunosensor arrays integrated with optic and electric detection, in the ng/L domain for herbicide in alimentation products and water by involving the microtechnology techniques on silicon and piezoceramic substrates and molecular biology techniques, accessible to project consortium.
The project will develop and involve technologies of the fabrication of immunosensor arrays integrated on semiconductor substrate (silicon) and piezoelectric substrate (langasite) for ultrarapide analyses with high accuracy, growth sensitivity (until 3 orders magnitude) comparative with ELISE (Enzyme–linked Immunosorbent Assay), actual immunoassay technique used and growth specificity detection to haptene, as: atrazine, hydroxy-atrazine, 2,6-dichloro-benzamide (BAM), 2,6-dichloro-benznitril (Dichlobenil).
The project presents a very complex degree, because integrate specific fabrication of the technology of the realization of microelectrodes with antibody and antigen deposition, specific reagents preparation and use the techniques to functionalisation of the surface for getting immunosensor arrays for herbicides detection.
For obtain the microelectrodes used like transducers for depositing biological material has to adaptation MEMS (Micro Electro Mechanical Systems) technologies, such as: realization 2D configuration and respective masks for microelectrodes structures, adaptation respective flux technologies for the depositing biological material on substrate correlative with the study of the reactive surface linkers for the adherence of biological material (antibody, antigen, marker) on substrate. Because, the characteristics of the answer of immunosensor, as: selectivity and sensitivity depend by the immobilisation and the linkage of the biocomponent to substrate, in principal, there are study two distinctive procedures for the functionalisation of the substrate (microelectrod) at the adherence of the bio-kit to this. There are two techniques studied, in parallel, for surface functionalisation: the organofunctionalisation method (organic compounds with: thiol (-SH) group, amino (-NH2) group, medium modifier is organic group) and inorganofunctionalisation method (inorganic compounds, the fixed group can be an organometalic compound or a metal (gold).